L. Senila, M. Miclean, E. Levei, C. Roman, C. Majdik, Preliminary results on the analysis of steroids in poultry samples by direct immersion solid-phase microextraction and gas chromatography-mass spectrometry, Studia Universitatis Babes-Boyai CHEMIA, 2 (2011) 107-114

Title: Preliminary results on the analysis of steroids in poultry samples by direct immersion solid-phase microextraction and gas chromatography-mass spectrometry

Abstract: This paper reports a rapid method for analysis of steroid hormones in poultry serum samples. The target compounds were first extracted by direct immersion on solid-phase microextraction (SPEME) procedure, followed by on fiber derivatization with methyl-N-(trimethylsilyl) trifluoroacetamide (MSTFA) and, final, analysis by gas chromatography with mass spectrometry (GC-MS). The average correlation coefficient of the calibration curve of the steroid hormones was 0.9955. The LOD/(LOQ) values of the steroid hormones in poultry serum samples were in the range of 0.020-0.068/(0.060-0.204) µg L^-1. The concentrations of estrone and ß-estradiol in poultry serum were 2.08 µg L^-1 and 3.61 µg L^-1, respectively.

Keywords: Steroid hormones, SPME, GC-MS