A. Gog, M. Chintoanu, M. Roman, E. Luca, C. Paizs, F. D. Irimie, Biodiesel production from sunflower oil with candida antarctica lipase B, Studia Chemia, 1 (2011) 71-80.

Title: Biodiesel production from sunflower oil with candida antarctica lipase B

ABSTRACT. In this paper, we have evaluated the efficacy of an enzymatic method for the transesterification process of sunflower oil with methanol in the presence of tert-butanol. tert-Butanol was used as the reaction medium to eliminate both negative effect caused by excessive methanol and glycerol resulted as by product. Using the following reaction conditions: 1% Novozym 435 based on oil weight, methanol / oil molar ratio 6:1, tert-butanol/ oil volume ratio 6:1, the FAME reached its maximum level after 3h, with a value of 78.6% (m/m).

Keywords: biodiesel, tert-Butanol, Novozym 435, enzymatic transesterification