M. A. Calin, R. M. Ion, The efficacy of photodynamic inactivation of the microorganisms using laser souces and methylene blue as photosensitizer, J. Optoelectron. Adv. Mater. 12/1 (2010) 141-145.

Title: The efficacy of photodynamic inactivation of the microorganisms using laser souces and methylene blue as photosensitizer

Abstract: The large spread of pathogen microorganisms in nature and their increasing resistence to drugs, lead to alternative treatment methods, more efficient and without microbial resistance. One of these methods is the photodynamic inactivation of microorganisms. In the present paper, we are discussing the efficacity of the laser system SCL (INOE 2000, P = 15 mW, λ = 635 nm) on yeast cells (Saccharomyces cerevisiae - as model system) treated with methylene blue as sensitizer. Diffuse reflectance spectroscopy and Kramers – Kronig analysis have been used for in vitro determination of the refractive index changes of yeast cells induced by laser irradiation.

Key words: Saccharomyces cerevisiae, photodynamic effect, photosensitizer, laser source, diffuse reflectance spectrometry, refractive index, microscopy