L. Senila, A. Gog, M. Senila, C. Roman, F. Irimie, Analysis of glucose obtained from wood carbohydrates by gas chromatography-mass spectrometry, Studia Chemia, 3 (2010) 51-56.

Title: Analysis of glucose obtained from wood carbohydrates by gas chromatography-mass spectrometry

Abstract: The aim of this study was the development and validation of a new method for carbohydrates analyses obtained from woody biomass, as a step of bioethanol production. In this study, we have quantified carbohydrates in wood by gas chromatography (GC) after derivatization. Quantification of carbohydrates was made by liquid-liquid extraction, oximation and silylation and, finally, analysis by gas chromatographymassspectrometry (GC-MS). Monosaccharide derivatives were identified by their GC retention time and MSfragmentation. N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) was used as derivatization reagent to prepare trimethylsilyl derivatives of hydrolysis products of cellulose and hemicellulose after pretreatmentand hydrolysis of wood. The carbohydrates were quantified by using salicin as internal standard.

Keywords: carbohydrates, wood, derivatization, GC-MS